Amakuru - umusaruro wa vanillin karemano mubikoresho bishoboka

I.ibicuruzwa

Vanillin nimwe mubintu bizwi cyane kandi bikoreshwa cyane kwisi. Ubusanzwe, byakuwe mu bishyimbo bya vanilla, bihenze kandi bihura n'ibibazo bijyanye no kuramba no gutanga urunigi. Ariko, hamwe niterambere ryibinyabuzima, cyane cyane murwego rwa biobial biotraformation, ibihe bishya kumusaruro wa Vanillin wagaragaye. Gukoresha mikorobe yo guhindura ibinyabuzima ibikoresho byifatizo mbisi byatanze inzira ifatika yubukungu kuri synthesis ya unillin. Ubu buryo ntabwo bukemura ibibazo birambye gusa ahubwo binatanga ibisubizo bishya byinganda zibikwiye. Ubushakashatsi bwakozwe n'ikigo cya siyansi n'ikoranabuhanga (SMMIT) byatanze ibisobanuro byuzuye kuri synthesio ya Vanillin no gusabana mu rwego rw'ibinyabuzima, mu ncamake mu nzego zitandukanye n'ibisabwa bitandukanye mu nganda.

II. Nigute ushobora kubona imiyoboro isanzwe kubikoresho bishobora kuvugururwa

Gukoresha aside Felique nka Substrate

Acide ya Frulike, akomoka ku masoko nka gar umuceri bran na oat bran, yerekana ko ibintu bisa na binyuramo kandi bikora nk'inzangano zikoreshwa cyane ku musaruro wa wanillin. Microorganism zitandukanye nka pseudomasi, aspergillus, stpectoms, kandi ibihumyo byahawe akazi ko gukora imisaruro ya billillin kuva aside Felique. Ikigaragara ni uko amoko nka Amycolatopsis n'uwuzura it yera yamenyekanye nk'abakandida bashobora gutanga imillin muri aside ya Frulin. Ubushakashatsi bwinshi bwakoze iperereza ku musaruro wa bilcillin wo muri aside Felique hakoreshejwe mikorobe, uburyo bworoshye, hamwe na sisitemu zidashira, kwerekana imiterere n'ubushobozi bw'ubu buryo.

Synthemasi ya enzymatique ya anillin kuva aside Felique irimo urufunguzo rwa enzyme Feruryl Esterase, niyagabanye hydrolysis ya ester acillin muri acillin nibindi bifitanye isano nibicuruzwa. Mugushakisha ubwinshi bwa vanillin biosythes enzymes muri sisitemu yubusa, abashakashatsi bateje imbere aside Felic (20mm) muri winjin (15mm). Byongeye kandi, imikoreshereze ya selile ya microbilisation yakwitayeho kubera biocompdatible nziza kandi ituze mubihe bitandukanye. Ubuhanga bwa tekinike yo kwizihiza bwa bilnillin kuva aside Felique yatejwe imbere, ikuraho ibikenewe coenzymes. Ubu buryo burimo igikoxyme-cyigenga deransaxylase na coenzyme-ogisijeni yigenga ishinzwe guhindura aside felique muri winjin. Co-idahuza na FDC na CSO2 ituma umusaruro wa 2.5 wa Vanillin wo muri aside Felique, harangaho urugero rwabapayiniya rutanga binyuze muri enzyme biotechnology.

Gukoresha EugenOl / isoeugenol nka substrate

Eugen na Isoeugenol, igihe yagejejwe kuri bioConversion, itanga imillin na metabolite ijyanye na Metabolite, zabonetse zifite agaciro kinyuranye nubukungu. Ubushakashatsi bwinshi bwakoze ubushakashatsi bwo gukoresha mikorobe yahinduwe kandi isanzwe igaragara muri stnthezes ya fagitillin muri Eugenol. Ubushobozi bwo gutesha agaciro Eugenol bwagaragaye muri bacteri na fungi, harimo ariko ntibigarukira kuri bacillus, Pseudomasi, Pseudomani, na Rhodococus, yerekana ubushobozi bwabo muri Eugenol - yakomokaga kuri wanillin-yavanyeho. Imikoreshereze ya EugenOl OXIDASE (Eugo) nka enzyme kumuntu wangiza wa unillin mubidukikije byagaragaje ubushobozi bukomeye. Eugo yerekana umutekano nigikorwa hejuru yumutwe mubi, hamwe nibikorwa Eugo igenda yiyongera no kugabanya igihe cyimyitwarire. Byongeye kandi, ikoreshwa rya Eugo ya Imbilize yemerera kugarura biocatast muri 18 react yimodoka, biganisha ku kwiyongera kwiyongera 12 mumusaruro wa biocatalyst. Mu buryo nk'ubwo, enzsulimed enzyme cso2 irashobora guteza imbere ihinduka rya isoeugenol muri wanillin ridashingiye kuri coenzymes.

Ibindi bisohoka

Usibye aside hamwe na Eugenol, ibindi bibanza nka aside ya vanillique na c6-c3 phenylpropanops byagaragaye nkibishobora gutanga umusaruro wa bilnillin. Acide ya facollic, yakozwe nkibicuruzwa byangiza bidasanzwe cyangwa nkibice birushaho guhangana ninzira ya metabolic, ifatwa nkinzangano za Vanillin zishingiye kuri bio. Byongeye kandi, itanga ubushishozi mu mikoreshereze ya C6-C3 Phenylpropanoids Synthesis ya Vanillin irerekana amahirwe adasanzwe ya prevour nziza kandi idashya.

Mu gusoza, gukoresha ibikoresho bishobora kongerwa ku musaruro wa vanillin kamere binyuze muri biobial Biotransiation ni iterambere ryingenzi mu nganda ziburinzi. Ubu buryo butanga ubundi buryo, burambye bwo gukora imiyoboro ya binllin, ikemura ibibazo birambye no kugabanya kwishingikiriza muburyo gakondo. Gusaba bitandukanye nubukungu bwa fagitills munganda zibiribwa bishimangira akamaro ko gukomeza gukora ubushakashatsi niterambere. Iterambere ry'ejo hazaza mu rwego rw'umusaruro wa Vanillin Usanzwe zifite ubushobozi bwo guhindura inganda zigezweho, gutanga ubundi buryo burambye kandi bw'incuti kuri flavor udushya. Mugihe dukomeje gukoresha ubushobozi bushobora kubaho ibikoresho bishobora kongerwa hamwe niterambere ryurubuga, umusaruro wa vanillin mubice bitandukanye byerekana inzira nziza yo gutangaza.

III. Ni izihe nyungu zo gukoresha umutungo ushobora kongwa kugirango ubyare imillin

Ishuti Ishuti:Gukoresha ibikoresho bishobora kongerwa nkibimera na biomass imyanda kugirango itange ibicaln, bikagabanya ingaruka mbi kubidukikije, kandi bigabanye imyuka ya gasutamo.

Kuramba:Gukoresha amikoro gishobora kuvugururwa bifasha gutanga ingufu n'ibikoresho bibisi, gufasha kurinda umutungo kamere kandi wujuje ibiza n'ibisekuruza bizaza.

Kurinda Ibinyabuzima:Binyuze mu gukoresha umutungo uko bivuguruzanya, ibikoresho byo mu gasozi birashobora kurindwa, bigira uruhare mu kurengera ibinyabuzima ndetse no kubungabunga ibidukikije.

Ubwiza bwibicuruzwa:Ugereranije na synthetic vanillin, vanillin karemano birashobora kugira inyungu nyinshi muburyo bwiza bwa aroma nubuzima karemano, bizafasha kuzamura ireme ryibicuruzwa nuburyo budahwitse.

Gabanya kwishingikiriza kubice byibinyabuzima:Gukoresha ibikoresho bishobora kongerwa bifasha kugabanya kwishingikiriza kuri friels yimbeba, bifitiye akamaro umutekano wingufu nimiterere ingufu zitandukanye. Twizere ko amakuru yavuzwe haruguru ashobora gusubiza ibibazo byawe. Niba ukeneye inyandiko yerekanwe mucyongereza, nyamuneka umbwire ko nshobora kuguha.

IV. Umwanzuro

Ubushobozi bwo gukoresha ibikoresho bishobokaburwa kugirango bigaragaze imiyoboro karemano nkubundi buryo burambye kandi bwangiza ibidukikije ni ngombwa. Ubu buryo bufite isezerano mugukemura ibibazo byiyongera kuri galllin karemano mugihe bigabanya kwishingikiriza muburyo bwumusaruro wa Sinter.

Ibicuruzwa bisanzwe bifata umwanya wingenzi munganda bukwiye, bifite agaciro kacyo biranga impumuro no gukoresha cyane nkumukozi mwiza mubicuruzwa bitandukanye. Ni ngombwa gushimangira akamaro ka pigillin karemano nkigikoresho cyashakishijwe mubiribwa, ibinyobwa, hamwe ninganda zihumura bitewe nubwumvikane bwihariye bwumvikana kandi ukunda flavour.

Byongeye kandi, umurima wa vanillin ya kamere usanzwe utanga amahirwe menshi yo gukomeza ubushakashatsi niterambere. Ibi bikubiyemo gushakisha ikoranabuhanga rishya hamwe nuburyo bushya bwo kuzamura imikorere no kuramba byo gukora imiyoboro isanzwe kubikoresho bishobora kongerwa. Byongeye kandi, iterambere ryuburyo bukomeye kandi buhendutse bwo gutanga umusaruro uzagira uruhare runini mu kurera imiyoboro isanzwe nkiyindi miterere irambye kandi yincuti yindirimbo.