Amakuru - Umusaruro Kamere ya Vanillin Uhereye kubikoresho bishya

I. Intangiriro

Vanillin ni kimwe mu bintu bizwi cyane kandi bikoreshwa cyane ku isi.Ubusanzwe, yakuwe mu bishyimbo bya vanilla, bihenze kandi bihura n’ibibazo bijyanye no kuramba no kutagira isoko.Ariko, hamwe niterambere ryibinyabuzima, cyane cyane mubijyanye na mikorobe ya biotransformation, hagaragaye ibihe bishya byumusaruro wa vanilline.Gukoresha mikorobe mu guhindura ibinyabuzima by’ibikoresho fatizo byatanze inzira ifatika mu bukungu bwo guhuza vanillin.Ubu buryo ntabwo bukemura ibibazo biramba gusa ahubwo butanga ibisubizo bishya mubikorwa byinganda.Ubushakashatsi bwakozwe n'ikigo cya SRM cy'ubumenyi n'ikoranabuhanga (SRMIST) cyatanze isuzuma ryimbitse ku buryo bwa elektiki ku bijyanye na synthesis biologiya ya vanillin hamwe n’ibikoreshwa mu rwego rw’ibiribwa, mu ncamake uburyo butandukanye bwo guhuza ibinyabuzima bya vanilline biva mu bice bitandukanye kandi bitandukanye. gusaba mu nganda zibiribwa.

II.Nigute Wabona Vanillin Kamere Mubikoresho Bisubirwamo

Gukoresha Acide Ferulic nka Substrate

Acide Ferulic, ikomoka kumasoko nkumuceri wumuceri na oat bran, yerekana imiterere isa na vanillin kandi ikora nka substrate ikoreshwa cyane mugukora vanillin.Ibinyabuzima bitandukanye nka Pseudomonas, Aspergillus, Streptomyces, na fungi byakoreshejwe mu gukora vanilline ikomoka kuri aside ferulike.Ikigaragara ni uko amoko nka Amycolatopsis na White-rot fungi yagaragaye nkabakandida bashobora gukora vanilline muri acide ferulic.Ubushakashatsi bwinshi bwakoze ubushakashatsi ku musaruro wa vanilline ukomoka kuri acide ferulic ukoresheje mikorobe, uburyo bwa enzymatique, hamwe na sisitemu yimibilisitiya, byerekana byinshi hamwe nubushobozi bwubu buryo.

Synthesis ya enzymatique ya vanilline ivuye muri acide ferulic ikubiyemo enzyme yingenzi feruloyl esterase, itera hydrolysis yumubano wa ester muri acide ferulic, ikarekura vanillin nibindi bifitanye isano nibicuruzwa.Mugushakisha ubwinshi bwimisemburo ya vanillin biosynthetic enzymes muri sisitemu idafite selile, abashakashatsi bakoze uburyo bwiza bwa recombinant Escherichia coli bushobora guhindura aside ferulike (20mM) muri vanilline (15mM).Byongeye kandi, gukoresha mikorobe ya immobilisation ya mikorobe byitabiriwe cyane kubera biocompatibilité nziza kandi ihamye mubihe bitandukanye.Hashyizweho uburyo bushya bwa immobilisation yubuhanga bwa vanillin ikomoka kuri acide ferulic, bivanaho coenzymes.Ubu buryo bukubiyemo decarboxylase yigenga ya coenzyme na ogisijene yigenga ya coenzyme ishinzwe guhindura aside ferulike muri vanilline.Kwishyira hamwe kwa FDC na CSO2 bifasha gukora mg 2,5 mg ya vanilline ikomoka kuri acide ferulic mu bihe icumi byerekana, ibyo bikaba urugero rwambere rwumusemburo wa vanillin ukoresheje enzyme ya biotechnologiya.

Gukoresha Eugenol / Isoeugenol nka Substrate

Eugenol na isoeugenol, iyo bikorewe bioconversion, bitanga vanilline hamwe na metabolite bifitanye isano nayo, byagaragaye ko ifite ibintu bitandukanye kandi bifite agaciro gakomeye mubukungu.Ubushakashatsi bwinshi bwakoze ubushakashatsi ku ikoreshwa rya mikorobe ihindagurika kandi isanzwe ibaho kugirango ikomatanye vanillin kuva eugenol.Ubushobozi bwo kwangirika kwa eugenol bwagaragaye muri bagiteri zitandukanye no mu bihumyo, harimo ariko ntibigarukira kuri Bacillus, Pseudomonas, Aspergillus, na Rhodococcus, byerekana ubushobozi bwabo mu musemburo wa vanilline ukomoka kuri eugenol.Gukoresha okiside ya eugenol (EUGO) nka enzyme yumusaruro wa vanilline mubidukikije byerekanye imbaraga zikomeye.EUGO yerekana ituze nibikorwa murwego rwagutse rwa pH, hamwe na EUGO ikemuka byongera ibikorwa kandi bigabanya igihe cyo kubyitwaramo.Byongeye kandi, ikoreshwa rya EUGO ryimuwe ryemerera kugarura ibinyabuzima bigera kuri 18 byikurikiranya, bigatuma umusaruro wikwirakwizwa wikubye inshuro 12.Mu buryo nk'ubwo, enzyme ya immobilized CSO2 irashobora guteza imbere ihinduka rya isoeugenol muri vanillin udashingiye kuri coenzymes.

Izindi Substrates

Usibye aside ferulic na eugenol, ibindi bikoresho nka acide ya vanillic na C6-C3 fenylpropanoide byagaragaye ko ari insimburangingo ishobora kubyara vanillin.Acide ya Vanillic, ikorwa nkibicuruzwa biva mu kwangirika kwa lignine cyangwa nkibigize guhatanira inzira ya metabolike, bifatwa nkibibanziriza umusaruro wa bioilline ikomoka kuri bio.Byongeye kandi, gutanga ubushishozi ku mikoreshereze ya C6-C3 phenylpropanoide ya synthesis ya vanillin itanga amahirwe adasanzwe yo guhanga uburyohe burambye kandi bushya.

Mu gusoza, gukoresha umutungo ushobora kongera umusaruro wa vanilline karemano binyuze muri mikorobe ya biotransformation ni iterambere ryibanze mu nganda ziryoha.Ubu buryo butanga ubundi buryo, burambye bwo gukora vanillin, gukemura ibibazo biramba no kugabanya gushingira kuburyo gakondo bwo kuvoma.Porogaramu zitandukanye n’agaciro k’ubukungu bya vanillin mu nganda z’ibiribwa bishimangira akamaro ko gukomeza ubushakashatsi n’iterambere muri uru rwego.Iterambere ry'ejo hazaza mu bijyanye n'umusaruro wa vanilline karemano ufite ubushobozi bwo guhindura inganda ziryoshye, zitanga ubundi buryo burambye kandi bwangiza ibidukikije bwo guhanga udushya.Mugihe dukomeje gukoresha ubushobozi bwumutungo ushobora kuvugururwa niterambere ryibinyabuzima, umusaruro wa vanilline karemano uva mubice bitandukanye byerekana inzira itanga icyizere cyo guhanga udushya.

III.Ni izihe nyungu zo gukoresha umutungo ushobora kubyara vanilline karemano

Ibidukikije byangiza ibidukikije:Gukoresha umutungo ushobora kuvugururwa nkibimera n’imyanda ya biomass kugirango ubyare vanilline birashobora kugabanya ibikenerwa n’ibicanwa by’ibinyabuzima, kugabanya ingaruka mbi ku bidukikije, no kugabanya ibyuka bihumanya ikirere.

Kuramba:Gukoresha umutungo ushobora kuvugururwa bituma habaho ingufu zirambye zingufu nibikoresho fatizo, bifasha kurinda umutungo kamere no guhaza ibisekuruza bizaza.

Kurinda urusobe rw'ibinyabuzima:Binyuze mu gukoresha neza umutungo ushobora kuvugururwa, umutungo w’ibimera wo mu gasozi urashobora kurindwa, bigira uruhare mu kurinda urusobe rw’ibinyabuzima no kubungabunga ibidukikije.

Ubwiza bwibicuruzwa:Ugereranije na vanilline yubukorikori, vanilline karemano irashobora kugira ibyiza byinshi mubwiza bwimpumuro nziza nibiranga kamere, bizafasha kuzamura ubwiza bwibicuruzwa bihumura neza.

Mugabanye gushingira ku bicanwa biva mu kirere:Gukoresha umutungo ushobora kuvugururwa bifasha kugabanya gushingira ku bicanwa bito bito, bifasha umutekano w’ingufu n’imiterere itandukanye.Twizere ko amakuru yavuzwe haruguru ashobora gusubiza ibibazo byawe.Niba ukeneye inyandiko yerekana mucyongereza, nyamuneka umbwire kugirango nshobore kuguha.

IV.Umwanzuro

Ubushobozi bwo gukoresha umutungo wongeyeho kubyara vanilline karemano nkibindi biramba kandi bitangiza ibidukikije ni ngombwa.Ubu buryo bufite isezerano mugukemura ikibazo gikenerwa na vanilline karemano mugihe hagabanijwe gushingira kubikorwa byubukorikori.

Kamere ya vanillin ifite umwanya wingenzi mu nganda zikora uburyohe, ihabwa agaciro kubera impumuro nziza iranga no gukoreshwa cyane nkibintu bihumura ibicuruzwa bitandukanye.Ni ngombwa gushimangira akamaro ka vanilline karemano nkibintu bishakishwa mu nganda z’ibiribwa, ibinyobwa, n’impumuro nziza bitewe n’imiterere y’ibyiyumvo byayo kandi bikunda abaguzi bakunda uburyohe bwa kamere.

Byongeye kandi, urwego rwumusaruro wa vanillin karemano rutanga amahirwe menshi yo gukomeza ubushakashatsi niterambere.Ibi birimo gushakisha tekinolojiya mishya hamwe nuburyo bushya bwo kuzamura imikorere no kuramba kubyara vanilline karemano ivuye mumitungo ishobora kuvugururwa.Byongeye kandi, guteza imbere uburyo bunini kandi buhendutse bwo gutanga umusaruro bizagira uruhare runini mugutezimbere kwinshi kwa vanilline karemano nkuburyo burambye kandi bwangiza ibidukikije munganda zikora uburyohe.